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90plus 納米粒度儀應用案例-26
文獻名：Zinc oxide particles induce inflammatory responses in vascular endothelial cells via NF-κB signaling
作者：Tsui-Chun Tsoua, Szu-Ching Yeha, Feng-Yuan Tsaia, Ho-Jane Lina, Tsun-Jen Chengb, How-Ran Chaoc, Lin-Ai Taid
aDivision of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan
bInstitute of Occupational Medicine and Industrial Hygiene, National Taiwan University, Taipei, Taiwan
cDepartment of Environmental Science and Engineering, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan
dCenter for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli County, Taiwan
摘要：This study investigated inflammatory effects of zinc oxide (ZnO) particles on vascular endothelial cells. The effects of 50 and 100-nm ZnO particles on human umbilical vein endothelial cells (HUVECs) were characterized by assaying cytotoxicity, cell proliferation, and glutathione levels. A marked drop in survival rate was observed when ZnO concentration was increased to 45μg/ml. ZnO concentrations of ≤3 μg/ml resulted in increased cell proliferation, while those of ≤45μg/ml caused dose-dependent increases in oxidized glutathione levels. Treatments with ZnO concentrations ≤45μg/ml were performed to determine the expression of intercellular adhesion molecule-1 (ICAM-1) protein, an indicator of vascular endothelium inflammation, revealing that ZnO particles induced a dose-dependent increase in ICAM-1 expression and marked increases in NF-κB reporter activity. Overexpression of IκBα completely inhibited ZnO-induced ICAM-1 expression, suggesting NF-κB plays a pivotal role in regulation of ZnO-induced inflammation in HUVECs. Additionally, TNF-α, a typical inflammatory cytokine, induced ICAM-1 expression in an NF-κB-dependent manner, and ZnO synergistically enhanced TNF-α-induced ICAM-1 expression. Both 50 and 100-nm ZnO particles agglomerated to similar size distributions. This study reveals an important role for ZnO in modulating inflammatory responses of vascular endothelial cells via NF-κB signaling, which could have important implications for treatments of vascular disease.
關鍵詞：Zinc oxide; Endothelium; Inflammation; NF-κB; Adhesion molecules; Cytokines
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